nuclear staining dye dapi (Beyotime)
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Nuclear Staining Dye Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nuclear+dye+dapi/DAPI/pmc12901475-70-1-5
Average 99 stars, based on 32109 article reviews
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Incubation:Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling. Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence. Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization. Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a Article Title: Nonvisual system-mediated body color change in fish reveals nonvisual function of Opsin 3 in skin. Article Snippet: Body-color changes in many poikilothermic animals can occur quickly.. This color change is generally initiated by visual system, followed by neuromuscular or neuroendocrine control.. We have previously showed that the ventral skin color of the large yellow croaker (Larimichthys crocea) presents golden yellow in dark environment and quickly changes to silvery white in light environment. Staining:Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling. Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence. Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a Protein Quantitation:Article Title: Microfluidic Device-Based In Vivo Detection of PD-L1-Positive Small Extracellular Vesicles and Its Application for Tumor Monitoring. Article Snippet: SU8-2050 and AZ9260 were purchased from AZ Electronic Materials Corp. PDMS and curing agent were purchased from GE Toshiba Silicones Co., Ltd., Japan. .. other:Article Title: Near-Infrared Imaging Agent ABSi-148 Alleviates CA IX-Mediated Hypoxic Fibrosis in Inflammation-Cancer Transition. Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) remains a formidable challenge due to its late diagnosis and intrinsic treatment resistance, exacerbates by its development from chronic inflammation to cancer transition (ICT).. Here, this investigation aims to develop and evaluate ABSi-148, a novel near-infrared (NIR) agent targeting hypoxic carbonic anhydrase IX (CA IX), for its potential applications in ICT imaging and even PDAC treatment.. ABSi-148 is synthesized from 4-(2-Aminoethyl) benzene sulfonamide (ABS), a sulfonamide derivative, conjugating with MHI-148 dye with merits of exceptional NIR-emitting traits, high biocompatibility, and deep tissue penetration imaging capability. Transfection:Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a Membrane:Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a Article Title: Three-Dimensional Magnetic Chip with Extracorporeal Circulation for Circulating Tumor Cell In Vivo Detection and Tumor Growth Inhibition Article Snippet: Hoechst 33342 was purchased from Invitrogen. .. Microscopy:Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization. Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a |
