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nuclear staining dye dapi  (Beyotime)


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    Structured Review

    Beyotime nuclear staining dye dapi
    Nuclear Staining Dye Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+dye+dapi/DAPI/pmc12901475-70-1-5
    Average 99 stars, based on 32109 article reviews
    nuclear staining dye dapi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling.
    Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence.

    Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development
    Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a nuclear dye DAPI (Beyotime, China) at 37°C for 10 min. After removing the DAPI solution, the cells were washed three times with PBS, mounted in an antifade mounting medium (Solarbio, China), and imaged using a Zeiss laser scanning confocal microscope (LSM880, 294 Carl Zeiss, Oberkochen, Germany). ..

    Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization
    Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a nuclear dye DAPI (Beyotime, China) for 5 min. Confocal images were acquired using a confocal microscope (Zeiss, LSM780, Germany). ..

    Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization.
    Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a nuclear dye DAPI (Beyotime, China) for 5 min. Confocal images were acquired using a confocal microscope (Zeiss, LSM780, Germany). ..

    Article Title: Nonvisual system-mediated body color change in fish reveals nonvisual function of Opsin 3 in skin.
    Article Snippet: Body-color changes in many poikilothermic animals can occur quickly.. This color change is generally initiated by visual system, followed by neuromuscular or neuroendocrine control.. We have previously showed that the ventral skin color of the large yellow croaker (Larimichthys crocea) presents golden yellow in dark environment and quickly changes to silvery white in light environment.

    Staining:

    Article Title: Kongensin A targeting PI3K attenuates inflammation-induced osteoarthritis by modulating macrophage polarization and alleviating inflammatory signaling.
    Article Snippet: The inflammatory microenvironment, polarization of macrophages towards the M1 phenotype, and consequent matrix degradation and senescence of chondrocytes are primary contributors to the degeneration of knee joint cartilage, further exacerbating the progression of osteoarthritis (OA).. Kongensin A (KA) is a recently identified natural plant extract exhibiting anti-necrotic apoptosis and anti-inflammatory properties, but the potential efficacy in alleviating OA remains uncertain.. The current research lucubrated the effect of KA on the inflammatory microenvironment and macrophage polarization, as well as its regulatory function in extracellular matrix (ECM) metabolism and chondrocyte senescence.

    Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development
    Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a nuclear dye DAPI (Beyotime, China) at 37°C for 10 min. After removing the DAPI solution, the cells were washed three times with PBS, mounted in an antifade mounting medium (Solarbio, China), and imaged using a Zeiss laser scanning confocal microscope (LSM880, 294 Carl Zeiss, Oberkochen, Germany). ..

    Protein Quantitation:

    Article Title: Microfluidic Device-Based In Vivo Detection of PD-L1-Positive Small Extracellular Vesicles and Its Application for Tumor Monitoring.
    Article Snippet: SU8-2050 and AZ9260 were purchased from AZ Electronic Materials Corp. PDMS and curing agent were purchased from GE Toshiba Silicones Co., Ltd., Japan. .. Nuclear dye DAPI, Hoechst 33342 and Enhanced BCA Protein Quantitation Kit were purchased from Beyotime Biotechnology. ..

    other:

    Article Title: Near-Infrared Imaging Agent ABSi-148 Alleviates CA IX-Mediated Hypoxic Fibrosis in Inflammation-Cancer Transition.
    Article Snippet: Pancreatic ductal adenocarcinoma (PDAC) remains a formidable challenge due to its late diagnosis and intrinsic treatment resistance, exacerbates by its development from chronic inflammation to cancer transition (ICT).. Here, this investigation aims to develop and evaluate ABSi-148, a novel near-infrared (NIR) agent targeting hypoxic carbonic anhydrase IX (CA IX), for its potential applications in ICT imaging and even PDAC treatment.. ABSi-148 is synthesized from 4-(2-Aminoethyl) benzene sulfonamide (ABS), a sulfonamide derivative, conjugating with MHI-148 dye with merits of exceptional NIR-emitting traits, high biocompatibility, and deep tissue penetration imaging capability.

    Transfection:

    Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development
    Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a nuclear dye DAPI (Beyotime, China) at 37°C for 10 min. After removing the DAPI solution, the cells were washed three times with PBS, mounted in an antifade mounting medium (Solarbio, China), and imaged using a Zeiss laser scanning confocal microscope (LSM880, 294 Carl Zeiss, Oberkochen, Germany). ..

    Membrane:

    Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development
    Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a nuclear dye DAPI (Beyotime, China) at 37°C for 10 min. After removing the DAPI solution, the cells were washed three times with PBS, mounted in an antifade mounting medium (Solarbio, China), and imaged using a Zeiss laser scanning confocal microscope (LSM880, 294 Carl Zeiss, Oberkochen, Germany). ..

    Article Title: Three-Dimensional Magnetic Chip with Extracorporeal Circulation for Circulating Tumor Cell In Vivo Detection and Tumor Growth Inhibition
    Article Snippet: Hoechst 33342 was purchased from Invitrogen. .. Nuclear dye DAPI, membrane dye DiI and DiO were purchased from Beyotime Biotechnology. .. D-Luciferin sodium salt was purchased from Shanghai yuanye Bio-Technology.

    Microscopy:

    Article Title: Molecular characterization of a short neuropeptide F signaling system in the swimming crab, Portunus trituberculatus, and its role in ovarian development
    Article Snippet: .. The transfected cells were fixed with 4% paraformaldehyde (PFA) for 20 min, and then stained with the cell membrane probe Dil (Beyotime, China) at 37°C for 10 min. After removing the Dil solution, the cells were washed three times with PBS and further incubated with a nuclear dye DAPI (Beyotime, China) at 37°C for 10 min. After removing the DAPI solution, the cells were washed three times with PBS, mounted in an antifade mounting medium (Solarbio, China), and imaged using a Zeiss laser scanning confocal microscope (LSM880, 294 Carl Zeiss, Oberkochen, Germany). ..

    Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization
    Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a nuclear dye DAPI (Beyotime, China) for 5 min. Confocal images were acquired using a confocal microscope (Zeiss, LSM780, Germany). ..

    Article Title: High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization.
    Article Snippet: For visualization, fluorescently labeled secondary antibodies (Alexa Fluor® 488, abcam, ab150113, USA) (1:800) were used for 1 h at 37 °C. .. Cells were further incubated with a nuclear dye DAPI (Beyotime, China) for 5 min. Confocal images were acquired using a confocal microscope (Zeiss, LSM780, Germany). ..



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    Image Search Results


    ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Journal: Science Advances

    Article Title: Multiregional profiling reveals THBS1 - SPP1 monocyte-macrophage axis drives immunosuppression and outcome in colorectal liver metastases

    doi: 10.1126/sciadv.aed1296

    Figure Lengend Snippet: ( A ) Graphs depicting main cluster interactors based on incoming and outgoing interaction strengths in the tumor edge (left) and in the tumor core (right). ( B ) Representative immunofluorescent staining (left) and corresponding spatial plots (right) of CD8 + T cells (CD8 + ), monocytes (Mono, CD14 + IBA1 − ), and MoMs (CD14 + IBA1 + ) in the tumor edge and tumor core. Scale bars, 50 μm. ( C ) SpatialScore calculated from immunofluorescent data per patient in tumor edge and tumor core. Error bars denotes means ± SEM. P values, Wilcoxon rank-sum test. ( D ) Heatmap depicting selected ligand-receptor interactions from T cell clusters to THBS1 + monocytes (top) and LR interactions from THBS1 + monocytes to T cell clusters (bottom), enriched in the tumor edge. ( E ) Heatmap depicting selected LR interactions from THBS1 + monocytes to T cell clusters, enriched in the tumor edge. ( F ) Scatter plot shows the correlation between the abundance of SPP1 + macrophages and exhausted CD8 + T (CD8 + Tex) cells in the CRLM microarray dataset GSE159216 ( n = 171). The error band indicates the 95% confidence interval. In (D) and (E), color intensity represents the probability of communication. DAPI, 4′,6-diamidino-2-phenylindole.

    Article Snippet: Sections were then incubated with primary antibodies at 4°C overnight followed by incubation with fluorophore-conjugated secondary antibodies (table S11) and nuclear dye 4′,6-diamidino-2-phenylindole (Thermo Fisher Scientific) at room temperature for 2 hours.

    Techniques: Staining, Microarray